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lactic acid bacteria lab strains  (ATCC)


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    ATCC lactic acid bacteria lab strains
    <t>LAB</t> BEVs exhibit anti‐inflammatory effects in vitro. A–D) Mouse RAW264.7 (A‐B) or human dTHP1 (C‐D) macrophages pretreated with BEVs display reduced inflammatory TNF‐α secretion following stimulation with 10 ng mL −1 LPS (for RAW264.7) or 250 ng mL −1 LPS + 20 ng mL −1 IFNγ (for dTHP1). Dosing (A–D): V.V. Low = 5E6 particles mL −1 , V. Low = 5E7 particles mL −1 , Low = 5E8 particles/ml, Med = 5E9 particles/ml, High = 5E10 particles mL −1 . E) RAW264.7 macrophages pretreated with BEVs from Gram‐negative probiotic EcN BEVs or Gram‐positive probiotic L. plantarum (Lp) BEVs followed by LPS stimulation; Dosing (E): Low = 1E9 particles mL −1 , High = 5E10 particles mL −1 . F) dTHP1 macrophages were treated with 5E9 particles/ml of BEVs that were previously covalently labeled with fluorescent Alexa Fluor 647;24 h later, cell uptake of fluorescently‐labeled BEVs was analyzed by flow cytometry. Controls included BEVs not labeled with Alexa Fluor 647 (unlabeled), PBS/Vehicle treated cells (No treat), or free dye (Alexa Fluor 647 carboxylate 10 um) G) dTHP1 viability 24 h after treatment with Lc, Lp, or EcN BEVs (2e11 particles mL −1 ) and LPS+IFNγ stimulation was assessed by CCK8 assay. Abbreviations in figures are as follows: Lp ( L. plantarum ), Lc ( L. paracasei ), Lre ( L. reuteri ), Lrh ( <t>L.</t> <t>rhamnosus</t> ), EcN ( E. coli Nissle 1917), DH5a ( E. coli DH5a), Dex (Dexamathosone). Statistical significance determined with one‐way ANOVA and Tukey post hoc test. If two groups share at least one letter, their means are not significantly different (α=0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars ± SD.
    Lactic Acid Bacteria Lab Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lactic acid bacteria lab strains - by Bioz Stars, 2026-08
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    Images

    1) Product Images from "Genetically‐Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model"

    Article Title: Genetically‐Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model

    Journal: Advanced Science

    doi: 10.1002/advs.202512679

    LAB BEVs exhibit anti‐inflammatory effects in vitro. A–D) Mouse RAW264.7 (A‐B) or human dTHP1 (C‐D) macrophages pretreated with BEVs display reduced inflammatory TNF‐α secretion following stimulation with 10 ng mL −1 LPS (for RAW264.7) or 250 ng mL −1 LPS + 20 ng mL −1 IFNγ (for dTHP1). Dosing (A–D): V.V. Low = 5E6 particles mL −1 , V. Low = 5E7 particles mL −1 , Low = 5E8 particles/ml, Med = 5E9 particles/ml, High = 5E10 particles mL −1 . E) RAW264.7 macrophages pretreated with BEVs from Gram‐negative probiotic EcN BEVs or Gram‐positive probiotic L. plantarum (Lp) BEVs followed by LPS stimulation; Dosing (E): Low = 1E9 particles mL −1 , High = 5E10 particles mL −1 . F) dTHP1 macrophages were treated with 5E9 particles/ml of BEVs that were previously covalently labeled with fluorescent Alexa Fluor 647;24 h later, cell uptake of fluorescently‐labeled BEVs was analyzed by flow cytometry. Controls included BEVs not labeled with Alexa Fluor 647 (unlabeled), PBS/Vehicle treated cells (No treat), or free dye (Alexa Fluor 647 carboxylate 10 um) G) dTHP1 viability 24 h after treatment with Lc, Lp, or EcN BEVs (2e11 particles mL −1 ) and LPS+IFNγ stimulation was assessed by CCK8 assay. Abbreviations in figures are as follows: Lp ( L. plantarum ), Lc ( L. paracasei ), Lre ( L. reuteri ), Lrh ( L. rhamnosus ), EcN ( E. coli Nissle 1917), DH5a ( E. coli DH5a), Dex (Dexamathosone). Statistical significance determined with one‐way ANOVA and Tukey post hoc test. If two groups share at least one letter, their means are not significantly different (α=0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars ± SD.
    Figure Legend Snippet: LAB BEVs exhibit anti‐inflammatory effects in vitro. A–D) Mouse RAW264.7 (A‐B) or human dTHP1 (C‐D) macrophages pretreated with BEVs display reduced inflammatory TNF‐α secretion following stimulation with 10 ng mL −1 LPS (for RAW264.7) or 250 ng mL −1 LPS + 20 ng mL −1 IFNγ (for dTHP1). Dosing (A–D): V.V. Low = 5E6 particles mL −1 , V. Low = 5E7 particles mL −1 , Low = 5E8 particles/ml, Med = 5E9 particles/ml, High = 5E10 particles mL −1 . E) RAW264.7 macrophages pretreated with BEVs from Gram‐negative probiotic EcN BEVs or Gram‐positive probiotic L. plantarum (Lp) BEVs followed by LPS stimulation; Dosing (E): Low = 1E9 particles mL −1 , High = 5E10 particles mL −1 . F) dTHP1 macrophages were treated with 5E9 particles/ml of BEVs that were previously covalently labeled with fluorescent Alexa Fluor 647;24 h later, cell uptake of fluorescently‐labeled BEVs was analyzed by flow cytometry. Controls included BEVs not labeled with Alexa Fluor 647 (unlabeled), PBS/Vehicle treated cells (No treat), or free dye (Alexa Fluor 647 carboxylate 10 um) G) dTHP1 viability 24 h after treatment with Lc, Lp, or EcN BEVs (2e11 particles mL −1 ) and LPS+IFNγ stimulation was assessed by CCK8 assay. Abbreviations in figures are as follows: Lp ( L. plantarum ), Lc ( L. paracasei ), Lre ( L. reuteri ), Lrh ( L. rhamnosus ), EcN ( E. coli Nissle 1917), DH5a ( E. coli DH5a), Dex (Dexamathosone). Statistical significance determined with one‐way ANOVA and Tukey post hoc test. If two groups share at least one letter, their means are not significantly different (α=0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars ± SD.

    Techniques Used: In Vitro, Labeling, Flow Cytometry, CCK-8 Assay

    LAB BEVs reduce severity in acute DSS‐induced colitis. Mice received BEVs (2.5E9 particles mouse −1 day −1 , oral gavage, Days 1–7). DSS (2.5%) was given in drinking water Days 0–6, then normal water Days 7–8 (washout). A) Body‐weight change relative to Day 0. B) Colon length at Day 8. C,D) Mesenteric lymph‐node CD4+ T‐cell populations/activation at endpoint: Treg (CD4+CD8−CD25+Foxp3+), Th17 (CD4+CD8−Foxp3−RORγt+), Naive (CD4+CD44−CD62L+), Effector (CD4+CD44+CD62L−), Central memory (CD4+CD44+CD62L+). E) qPCR array of human UC–associated genes in proximal colon, shown as log2 fold‐change versus sham (vehicle/PBS) colitis. F) Pearson correlation of colitis‐associated genes comparing treatment groups with naïve/healthy mice; R^2 closer to 1 indicates greater similarity to naive. G) RT‐qPCR of selected colitis genes in pooled, bulk colon RNA. Abbreviations: L. plantarum (Lp), L. paracasei (Lc), L. reuteri (Lre), L. rhamnosus (Lrh), E. coli Nissle 1917 (EcN), E. coli DH5a (DH5a). Statistics: one‐way ANOVA with Tukey post hoc; different letters p < 0.05. If two groups share at least one letter, their means are not significantly different (α = 0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant.
    Figure Legend Snippet: LAB BEVs reduce severity in acute DSS‐induced colitis. Mice received BEVs (2.5E9 particles mouse −1 day −1 , oral gavage, Days 1–7). DSS (2.5%) was given in drinking water Days 0–6, then normal water Days 7–8 (washout). A) Body‐weight change relative to Day 0. B) Colon length at Day 8. C,D) Mesenteric lymph‐node CD4+ T‐cell populations/activation at endpoint: Treg (CD4+CD8−CD25+Foxp3+), Th17 (CD4+CD8−Foxp3−RORγt+), Naive (CD4+CD44−CD62L+), Effector (CD4+CD44+CD62L−), Central memory (CD4+CD44+CD62L+). E) qPCR array of human UC–associated genes in proximal colon, shown as log2 fold‐change versus sham (vehicle/PBS) colitis. F) Pearson correlation of colitis‐associated genes comparing treatment groups with naïve/healthy mice; R^2 closer to 1 indicates greater similarity to naive. G) RT‐qPCR of selected colitis genes in pooled, bulk colon RNA. Abbreviations: L. plantarum (Lp), L. paracasei (Lc), L. reuteri (Lre), L. rhamnosus (Lrh), E. coli Nissle 1917 (EcN), E. coli DH5a (DH5a). Statistics: one‐way ANOVA with Tukey post hoc; different letters p < 0.05. If two groups share at least one letter, their means are not significantly different (α = 0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant.

    Techniques Used: Activation Assay, Quantitative RT-PCR



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    <t>LAB</t> BEVs exhibit anti‐inflammatory effects in vitro. A–D) Mouse RAW264.7 (A‐B) or human dTHP1 (C‐D) macrophages pretreated with BEVs display reduced inflammatory TNF‐α secretion following stimulation with 10 ng mL −1 LPS (for RAW264.7) or 250 ng mL −1 LPS + 20 ng mL −1 IFNγ (for dTHP1). Dosing (A–D): V.V. Low = 5E6 particles mL −1 , V. Low = 5E7 particles mL −1 , Low = 5E8 particles/ml, Med = 5E9 particles/ml, High = 5E10 particles mL −1 . E) RAW264.7 macrophages pretreated with BEVs from Gram‐negative probiotic EcN BEVs or Gram‐positive probiotic L. plantarum (Lp) BEVs followed by LPS stimulation; Dosing (E): Low = 1E9 particles mL −1 , High = 5E10 particles mL −1 . F) dTHP1 macrophages were treated with 5E9 particles/ml of BEVs that were previously covalently labeled with fluorescent Alexa Fluor 647;24 h later, cell uptake of fluorescently‐labeled BEVs was analyzed by flow cytometry. Controls included BEVs not labeled with Alexa Fluor 647 (unlabeled), PBS/Vehicle treated cells (No treat), or free dye (Alexa Fluor 647 carboxylate 10 um) G) dTHP1 viability 24 h after treatment with Lc, Lp, or EcN BEVs (2e11 particles mL −1 ) and LPS+IFNγ stimulation was assessed by CCK8 assay. Abbreviations in figures are as follows: Lp ( L. plantarum ), Lc ( L. paracasei ), Lre ( L. reuteri ), Lrh ( <t>L.</t> <t>rhamnosus</t> ), EcN ( E. coli Nissle 1917), DH5a ( E. coli DH5a), Dex (Dexamathosone). Statistical significance determined with one‐way ANOVA and Tukey post hoc test. If two groups share at least one letter, their means are not significantly different (α=0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars ± SD.
    Lactic Acid Bacteria Strains Against Escherichia Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    <t>LAB</t> BEVs exhibit anti‐inflammatory effects in vitro. A–D) Mouse RAW264.7 (A‐B) or human dTHP1 (C‐D) macrophages pretreated with BEVs display reduced inflammatory TNF‐α secretion following stimulation with 10 ng mL −1 LPS (for RAW264.7) or 250 ng mL −1 LPS + 20 ng mL −1 IFNγ (for dTHP1). Dosing (A–D): V.V. Low = 5E6 particles mL −1 , V. Low = 5E7 particles mL −1 , Low = 5E8 particles/ml, Med = 5E9 particles/ml, High = 5E10 particles mL −1 . E) RAW264.7 macrophages pretreated with BEVs from Gram‐negative probiotic EcN BEVs or Gram‐positive probiotic L. plantarum (Lp) BEVs followed by LPS stimulation; Dosing (E): Low = 1E9 particles mL −1 , High = 5E10 particles mL −1 . F) dTHP1 macrophages were treated with 5E9 particles/ml of BEVs that were previously covalently labeled with fluorescent Alexa Fluor 647;24 h later, cell uptake of fluorescently‐labeled BEVs was analyzed by flow cytometry. Controls included BEVs not labeled with Alexa Fluor 647 (unlabeled), PBS/Vehicle treated cells (No treat), or free dye (Alexa Fluor 647 carboxylate 10 um) G) dTHP1 viability 24 h after treatment with Lc, Lp, or EcN BEVs (2e11 particles mL −1 ) and LPS+IFNγ stimulation was assessed by CCK8 assay. Abbreviations in figures are as follows: Lp ( L. plantarum ), Lc ( L. paracasei ), Lre ( L. reuteri ), Lrh ( <t>L.</t> <t>rhamnosus</t> ), EcN ( E. coli Nissle 1917), DH5a ( E. coli DH5a), Dex (Dexamathosone). Statistical significance determined with one‐way ANOVA and Tukey post hoc test. If two groups share at least one letter, their means are not significantly different (α=0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars ± SD.
    Probiotic Lactic Acid Bacteria Labs Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lactic+acid+bacteria+strains/Bacteria/pmc12326657-86-10-24
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    LAB BEVs exhibit anti‐inflammatory effects in vitro. A–D) Mouse RAW264.7 (A‐B) or human dTHP1 (C‐D) macrophages pretreated with BEVs display reduced inflammatory TNF‐α secretion following stimulation with 10 ng mL −1 LPS (for RAW264.7) or 250 ng mL −1 LPS + 20 ng mL −1 IFNγ (for dTHP1). Dosing (A–D): V.V. Low = 5E6 particles mL −1 , V. Low = 5E7 particles mL −1 , Low = 5E8 particles/ml, Med = 5E9 particles/ml, High = 5E10 particles mL −1 . E) RAW264.7 macrophages pretreated with BEVs from Gram‐negative probiotic EcN BEVs or Gram‐positive probiotic L. plantarum (Lp) BEVs followed by LPS stimulation; Dosing (E): Low = 1E9 particles mL −1 , High = 5E10 particles mL −1 . F) dTHP1 macrophages were treated with 5E9 particles/ml of BEVs that were previously covalently labeled with fluorescent Alexa Fluor 647;24 h later, cell uptake of fluorescently‐labeled BEVs was analyzed by flow cytometry. Controls included BEVs not labeled with Alexa Fluor 647 (unlabeled), PBS/Vehicle treated cells (No treat), or free dye (Alexa Fluor 647 carboxylate 10 um) G) dTHP1 viability 24 h after treatment with Lc, Lp, or EcN BEVs (2e11 particles mL −1 ) and LPS+IFNγ stimulation was assessed by CCK8 assay. Abbreviations in figures are as follows: Lp ( L. plantarum ), Lc ( L. paracasei ), Lre ( L. reuteri ), Lrh ( L. rhamnosus ), EcN ( E. coli Nissle 1917), DH5a ( E. coli DH5a), Dex (Dexamathosone). Statistical significance determined with one‐way ANOVA and Tukey post hoc test. If two groups share at least one letter, their means are not significantly different (α=0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars ± SD.

    Journal: Advanced Science

    Article Title: Genetically‐Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model

    doi: 10.1002/advs.202512679

    Figure Lengend Snippet: LAB BEVs exhibit anti‐inflammatory effects in vitro. A–D) Mouse RAW264.7 (A‐B) or human dTHP1 (C‐D) macrophages pretreated with BEVs display reduced inflammatory TNF‐α secretion following stimulation with 10 ng mL −1 LPS (for RAW264.7) or 250 ng mL −1 LPS + 20 ng mL −1 IFNγ (for dTHP1). Dosing (A–D): V.V. Low = 5E6 particles mL −1 , V. Low = 5E7 particles mL −1 , Low = 5E8 particles/ml, Med = 5E9 particles/ml, High = 5E10 particles mL −1 . E) RAW264.7 macrophages pretreated with BEVs from Gram‐negative probiotic EcN BEVs or Gram‐positive probiotic L. plantarum (Lp) BEVs followed by LPS stimulation; Dosing (E): Low = 1E9 particles mL −1 , High = 5E10 particles mL −1 . F) dTHP1 macrophages were treated with 5E9 particles/ml of BEVs that were previously covalently labeled with fluorescent Alexa Fluor 647;24 h later, cell uptake of fluorescently‐labeled BEVs was analyzed by flow cytometry. Controls included BEVs not labeled with Alexa Fluor 647 (unlabeled), PBS/Vehicle treated cells (No treat), or free dye (Alexa Fluor 647 carboxylate 10 um) G) dTHP1 viability 24 h after treatment with Lc, Lp, or EcN BEVs (2e11 particles mL −1 ) and LPS+IFNγ stimulation was assessed by CCK8 assay. Abbreviations in figures are as follows: Lp ( L. plantarum ), Lc ( L. paracasei ), Lre ( L. reuteri ), Lrh ( L. rhamnosus ), EcN ( E. coli Nissle 1917), DH5a ( E. coli DH5a), Dex (Dexamathosone). Statistical significance determined with one‐way ANOVA and Tukey post hoc test. If two groups share at least one letter, their means are not significantly different (α=0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars ± SD.

    Article Snippet: All lactic acid bacteria (LAB) strains were obtained through ATCC ( Lacticaseibacillus rhamnosus GG (ATCC 53 103), Lacticaseibacillus paracasei (ATCC 334), Limosilactobacillus reuteri F 275 (ATCC 23 272), L. plantarum WCFS1 (ATCC BAA‐793).

    Techniques: In Vitro, Labeling, Flow Cytometry, CCK-8 Assay

    LAB BEVs reduce severity in acute DSS‐induced colitis. Mice received BEVs (2.5E9 particles mouse −1 day −1 , oral gavage, Days 1–7). DSS (2.5%) was given in drinking water Days 0–6, then normal water Days 7–8 (washout). A) Body‐weight change relative to Day 0. B) Colon length at Day 8. C,D) Mesenteric lymph‐node CD4+ T‐cell populations/activation at endpoint: Treg (CD4+CD8−CD25+Foxp3+), Th17 (CD4+CD8−Foxp3−RORγt+), Naive (CD4+CD44−CD62L+), Effector (CD4+CD44+CD62L−), Central memory (CD4+CD44+CD62L+). E) qPCR array of human UC–associated genes in proximal colon, shown as log2 fold‐change versus sham (vehicle/PBS) colitis. F) Pearson correlation of colitis‐associated genes comparing treatment groups with naïve/healthy mice; R^2 closer to 1 indicates greater similarity to naive. G) RT‐qPCR of selected colitis genes in pooled, bulk colon RNA. Abbreviations: L. plantarum (Lp), L. paracasei (Lc), L. reuteri (Lre), L. rhamnosus (Lrh), E. coli Nissle 1917 (EcN), E. coli DH5a (DH5a). Statistics: one‐way ANOVA with Tukey post hoc; different letters p < 0.05. If two groups share at least one letter, their means are not significantly different (α = 0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant.

    Journal: Advanced Science

    Article Title: Genetically‐Programmed Hypervesiculation of Lactiplantibacillus Plantarum Increases Production of Bacterial Extracellular Vesicles with Therapeutic Efficacy in a Preclinical Inflammatory Bowel Disease Model

    doi: 10.1002/advs.202512679

    Figure Lengend Snippet: LAB BEVs reduce severity in acute DSS‐induced colitis. Mice received BEVs (2.5E9 particles mouse −1 day −1 , oral gavage, Days 1–7). DSS (2.5%) was given in drinking water Days 0–6, then normal water Days 7–8 (washout). A) Body‐weight change relative to Day 0. B) Colon length at Day 8. C,D) Mesenteric lymph‐node CD4+ T‐cell populations/activation at endpoint: Treg (CD4+CD8−CD25+Foxp3+), Th17 (CD4+CD8−Foxp3−RORγt+), Naive (CD4+CD44−CD62L+), Effector (CD4+CD44+CD62L−), Central memory (CD4+CD44+CD62L+). E) qPCR array of human UC–associated genes in proximal colon, shown as log2 fold‐change versus sham (vehicle/PBS) colitis. F) Pearson correlation of colitis‐associated genes comparing treatment groups with naïve/healthy mice; R^2 closer to 1 indicates greater similarity to naive. G) RT‐qPCR of selected colitis genes in pooled, bulk colon RNA. Abbreviations: L. plantarum (Lp), L. paracasei (Lc), L. reuteri (Lre), L. rhamnosus (Lrh), E. coli Nissle 1917 (EcN), E. coli DH5a (DH5a). Statistics: one‐way ANOVA with Tukey post hoc; different letters p < 0.05. If two groups share at least one letter, their means are not significantly different (α = 0.05). If they share no letters, they are significantly different ( p < 0.05). For example, “a” versus “ab” = not significant; “a” versus “b” = significant.

    Article Snippet: All lactic acid bacteria (LAB) strains were obtained through ATCC ( Lacticaseibacillus rhamnosus GG (ATCC 53 103), Lacticaseibacillus paracasei (ATCC 334), Limosilactobacillus reuteri F 275 (ATCC 23 272), L. plantarum WCFS1 (ATCC BAA‐793).

    Techniques: Activation Assay, Quantitative RT-PCR